Applied Microbiology & Laboratory Techniques

TARGET AUDIENCE?

• This training is designed for:

Process and production staff involved in microbiological testing .
Laboratory analysts and technicians .
Microbiologists and research scientists.
Environmental testing personnel.
Quality control (QC) staff in pharmaceutical and food industries .

PROGRAM OBJECTIVES:

BY THE END OF THIS TRAINING, PARTICIPANTS WILL BE ABLE TO:

1- UNDERSTAND AND APPLY CULTURE MEDIA TYPES, PREPARATION, AND ASEPTIC TECHNIQUES.
2- PERFORM BACTERIAL CULTURING METHODS (STREAKING, SPREADING, POUR PLATES).
3- INTERPRET BLOOD AGAR HEMOLYSIS PATTERNS FOR CLINICAL USE.
4- CONDUCT AEROBIC PLATE COUNT (APC) AND MICROBIAL LIMIT TESTS (MLT).
5- DETECT AND IDENTIFY PATHOGENS USING MICROBIOLOGICAL AND BIOCHEMICAL METHODS.
6- PERFORM MICROBIOLOGICAL WATER ANALYSIS.
7- APPLY STAINING TECHNIQUES AND BASIC MICROSCOPY.
8- UNDERSTAND ELISA PRINCIPLES, WORKFLOW, AND APPLICATIONS.

OUTLINE TOPICS :

1. TYPES OF PHARMACEUTICAL WATER PURIFIED WATER (PW) WATER FOR INJECTION (WFI).
2. WATER SYSTEM COMPONENTS PRE-TREATMENT RO / EDI STORAGE TANKS DISTRIBUTION LOOPS.
3. GMP REQUIREMENTS MICROBIAL CONTROL BIOFILM PREVENTION.
4. VALIDATION & QUALIFICATION IQ / OQ / PQ SAMPLING PLANS.
5. TROUBLESHOOTING CONTAMINATION CASES SYSTEM FAILURES.

TRAINING METHODOLOGY:

• The training will include:

1- TECHNICAL PRESENTATIONS.
2- INTERACTIVE DISCUSSIONS AND Q&A SESSIONS.
3- HANDS-ON LABORATORY EXERCISES.
4- CASE STUDIES AND PROBLEM-SOLVING SCENARIOS.
5- VIDEO DEMONSTRATIONS OF LABORATORY TECHNIQUES.
6- PRACTICAL EXERCISES ON CULTURE MEDIA PREPARATION, MICROBIAL TESTING, AND ELISA.

TRAINING MODULES:

SEC 1- CULTURE MEDIA IN THE LABORATORY.

1- Types of culture media: general, selective, differential, enriched.

2- Composition and function of each type.

3- Commonly used media in microbiology laboratories.

SEC 2- MEDIA PREPARATION & BACTERIAL CULTURING TECHNIQUES.

Overview Of Common Water Purification Technologies:

1- Preparation Of Culture Media: Weighing, Mixing, Sterilization.

2- Aseptic Techniques.

3- Inoculation Methods: Streaking, Spreading, Pour Plate.

4- Incubation Conditions And Requirements.

SEC 3- BLOOD AGAR APPLICATIONS.

1- Composition Of Blood Agar.

2- Hemolysis Patterns: Alpha, Beta, Gamma.

3- Clinical And Diagnostic Significance.

SEC 4- AEROBIC PLATE COUNT (APC).

1- Principle and importance.

2- Procedure and calculation of CFU.

3- Interpretation of results in food and environmental samples.

SEC 5- DETECTION OF PATHOGENS IN SAMPLES.

1- Isolation And Identification Techniques.

2- Biochemical Tests Overview.

3- Common Pathogens And Their Significance.

SEC 6- MICROBIAL LIMIT TEST.

1- Purpose In Pharmaceutical And Food Industries.

2- Total Viable Count.

3- Tests For Specified Microorganisms.

4- Acceptance Criteria.

SEC 7- MICROBIOLOGICAL WATER ANALYSIS.

1- Indicator Organisms.

2- Membrane Filtration And Pour Plate Techniques.

3- Standards For Water Quality.

SEC 8- STAINING TECHNIQUES.

1- Gram Staining Principle And Interpretation.

2- Special Stains: Acid-Fast, Spore Stain.

3- Basics Of Microscopy.

SEC 9- ELISA PRINCIPLES.

1- Types Of ELISA: Direct, Indirect, Sandwich, Competitive.

2- Principle And Workflow.

3- Applications In Diagnostics And Research.

EXPECTED OUTCOMES:

PARTICIPANTS WILL:

GAIN PRACTICAL SKILLS IN LABORATORY MICROBIOLOGY.
BE ABLE TO PERFORM MICROBIAL TESTS ACCURATELY IN COMPLIANCE WITH GMP.
UNDERSTAND PATHOGEN DETECTION AND WATER TESTING PROTOCOLS.
APPLY STAINING AND ELISA TECHNIQUES IN RESEARCH AND DIAGNOSTIC LABS.
IMPROVE OVERALL QUALITY CONTROL AND LABORATORY EFFICIENCY.

TRAINING DELIVERABLES:

PARTICIPANTS WILL RECEIVE:

TRAINING PRESENTATION MATERIALS (PDF).
LABORATORY MANUALS AND SOP TEMPLATES.
PRACTICAL EXERCISE GUIDES.
CASE STUDIES AND EXAMPLE CALCULATIONS.
CERTIFICATE OF COMPLETION.

TRAINING CERTIFICATION:

PARTICIPANTS WHO COMPLETE THE PROGRAM WILL RECEIVE A CERTIFICATE OF COMPLETION IN APPLIED MICROBIOLOGY & LABORATORY TECHNIQUE.

TOTAL DURATION:

3 - 4 Days

Speak to a training specialist

Applied Microbiology & Laboratory Techniques.